A technique tha will be used in my project is the Sodium Dodecyl Sulfate- Poly-Acrilamide Gel Electrophoresis (SDS-PAGE). This technique consists in the separation of a protein sample that migrates through a porous matrix that controls the migration of its components according to their sizes when exposed through an electric field. The protein sample is treated previously with SDS that unfolds proteins by hrydrophobic interactions and gives them a net negative charge proportional to its size. This allows the multiple components of a protein extract to migrate at different rates according to their sizes that may be visualized by Coomasie Blue staining. This technique will help us to identify differential expression on two samples treated at different conditions.
As of today, my project has being in the process of design, preparation of lab equipment, ordering, and the wait for the materials neccesary for the experiment. In a scale of 1 through 5 the progress would be at 2. Since I’m part of a young microbiology Lab in a chemical engineering department, to have the neccesary materials and equipment to perform biological samples has been an arduos process. However, this has provided ample time for establishing a method based on published literature in order to reach our project goals.
